Development of diagnostic methods of Peach Latent Mosaic Viroid (PLMVd)
https://doi.org/10.69536/FKR.2021.27.95.001
Abstract
Peach latent mosaic viroid (PLMVd) is a quarantine pest included in the Common List of Quarantine Objects of the Eurasian Economic Union, the list of quarantine pests absent in the Eurasian Economic Union.
PLMVd affects stone fruit crops, causing significant yield losses. Methods for the detection and identification of this pathogen in the Russian Federation were developed by the specialists of FGBU “VNIIKR” in 2015–2018.
The article presents the results of testing and valid ation of several pairs of species-specific primers used in world practice for the diagnosis of PLMVd by the polymerase chain reaction (PCR) method, as well as the results of experiments with primers developed by the authors.
About the Authors
Yu. N. Prikhodko
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Yuri Prikhodko - PhD in Agriculture, leading re-searcher, Scientific and Methodological Department of Virology and Bacteriology, FGBU VNIIKR.
Bykovo, Ramenskoye, Moscow Oblast
T. S. Zhivaeva
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Tatiana Zhivaeva - researcher, Scientific and Methodological Department of Virology and Bacteriology, FGBU VNIIKR.
Bykovo, Ramenskoye, Moscow Oblast
E. N. Lozovaya
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Evgeniya Lozovaya - researcher, Postgraduate Studies Department.
Bykovo, Ramenskoye, Moscow Oblast
Yu. A. Shneyder
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Yuri Shneyder - PhD in Biology, Head of Research and Methodology and Experimental Center, Leading Researcher, FGBU VNIIKR.
Bykovo, Ramenskoye, Moscow Oblast
E. V. Karimova
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Elena Karimova - PhD in Biology, Head of Scienti-fic and Methodological Department of Virology and Bacteriology, FGBU VNIIKR.
Bykovo, Ramenskoye, Moscow Oblast
References
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Review
For citations:
Prikhodko Yu.N., Zhivaeva T.S., Lozovaya E.N., Shneyder Yu.A., Karimova E.V. Development of diagnostic methods of Peach Latent Mosaic Viroid (PLMVd). Plant Health and Quarantine. 2021;(3):47-57. https://doi.org/10.69536/FKR.2021.27.95.001
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