Diagnostics of Pepino mosaic virus (PepMV)
https://doi.org/10.69536/3725.2024.54.83.003
Abstract
Pepino mosaic virus (PepMV), a representative of the genus Potexvirus, is a quarantine pest for the EAEU and the EPPO countries, as well as some other countries on different continents. The main host plants of the virus are tomato and eggplant, though it also affects peppers, potatoes and pepino. Tomato fruit yield losses as a result of PepMV infection can reach 30-40%. PepMV is characterized by various pathways and can maintain in plant debris, soil, water, hydroponic solutions and on various inert surfaces for a long time. Seed infection plays an important role in the spread of PepMV. With infected seeds, the virus can spread to new territories, and single infected seeds in a batch can cause colossal damage to vegetable producers during the growing season. The global PepMV population consists of five genetically distinct strains, making it difficult to diagnose using molecular methods. The purpose of the research was to search for primers that allow universal detection of isolates of all PepMV strains and primers that allow highly specific determination of the strain affiliation of the detected isolates of this virus. Based on the results of testing 13 pairs of primers, three pairs of primers were identified for the universal detection of all PepMV strains and two pairs of primers for the highly specific detection of Eu/Peruvian strains. The need for further screening of primers for highly specific determination of CH1/US1 and CH2 PepMV strains has been stated.
About the Authors
Tatiana S. Zhivaeva
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Tatiana Zhivaeva, Researcher, Research and Methodology Department of Virology and Bacteriology,
Bykovo, Ramenskoye, Moscow Oblast, 140150.
Maria A. Pruchkina
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Maria Pruchkina, PhD student, Agronomist of Pest Risk and International Cooperation Department,
Bykovo, Ramenskoye, Moscow Oblast, 140150.
Yuri N. Prikhodko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuri Prikhodko, PhD in Agriculture, Leading Researcher, Research and Methodology Department of Virology and Bacteriology,
Bykovo, Ramenskoye, Moscow Oblast, 140150.
Yuri A. Shneyder
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuri Shneyder, PhD in Biology, Head of Research and Methodology Department of Virology and Bacteriology,
Bykovo, Ramenskoye, Moscow Oblast, 140150.
Elena V. Karimova
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Elena Karimova, PhD in Biology, Senior Researcher, Head of Research and Methodology Department of Virology and Bacteriology,
Bykovo, Ramenskoye, Moscow Oblast, 140150.
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Review
For citations:
Zhivaeva T.S., Pruchkina M.A., Prikhodko Yu.N., Shneyder Yu.A., Karimova E.V. Diagnostics of Pepino mosaic virus (PepMV). Plant Health and Quarantine. 2024;(2):41-52. https://doi.org/10.69536/3725.2024.54.83.003
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