phytosanitaryФитосанитария. Карантин растенийPlant Health and Quarantine2782-327X10.69536/3725.2024.54.83.003phytosanitary-160Research ArticleДИАГНОСТИКАDIAGNOSTICSДиагностика вируса мозаики пепино (PepMV)Diagnostics of Pepino mosaic virus (PepMV)ЖиваеваТ. С.ZhivaevaTatiana S.

Живаева Татьяна Степановна, научный сотрудник научно-методического отдела вирусологии,

р. п. Быково, г. о. Раменский, Московская обл., 140150.

Tatiana Zhivaeva, Researcher, Research and Methodology Department of Virology and Bacteriology,

Bykovo, Ramenskoye, Moscow Oblast, 140150.

zhivaeva.vniikr@mail.ruПручкинаМ. А.PruchkinaMaria A.

Пручкина Мария Александровна, аспирант, агроном отдела фитосанитарных рисков и международного взаимодействия,

р. п. Быково, г. о. Раменский, Московская обл., 140150.

Maria Pruchkina, PhD student, Agronomist of Pest Risk and International Cooperation Department,

Bykovo, Ramenskoye, Moscow Oblast, 140150.

anadiamena@gmail.comПриходькоЮ. Н.PrikhodkoYuri N.

Приходько Юрий Николаевич, кандидат сельскохозяйственных наук, ведущий научный сотрудник научно-методического отдела вирусологии,

р. п. Быково, г. о. Раменский, Московская обл., 140150.

Yuri Prikhodko, PhD in Agriculture, Leading Researcher, Research and Methodology Department of Virology and Bacteriology,

Bykovo, Ramenskoye, Moscow Oblast, 140150.

prihodko_yuri59@mail.ruhttps://orcid.org/0000-0002-7565-1241ШнейдерЮ. А.ShneyderYuri A.

Шнейдер Юрий Андреевич, кандидат биологических наук, начальник научно-методического отдела вирусологии, ведущий научный сотрудник,

р. п. Быково, г. о. Раменский, Московская обл., 140150.

Yuri Shneyder, PhD in Biology, Head of Research and Methodology Department of Virology and Bacteriology,

Bykovo, Ramenskoye, Moscow Oblast, 140150.

yury.shneyder@mail.ruhttps://orcid.org/0000-0001-6474-8913КаримоваЕ. В.KarimovaElena V.

Каримова Елена Владимировна, кандидат биологических наук, старший научный сотрудник, начальник научно-методического отдела вирусологии и бактериологии,

р. п. Быково, г. о. Раменский, Московская обл., 140150.

Elena Karimova, PhD in Biology, Senior Researcher, Head of Research and Methodology Department of Virology and Bacteriology,

Bykovo, Ramenskoye, Moscow Oblast, 140150.

elenavkar@mail.ruФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»)РоссияFGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)Russian Federation202429052024024152Copyright © Живаева Т.С., Пручкина М.А., Приходько Ю.Н., Шнейдер Ю.А., Каримова Е.В., 20242024Живаева Т.С., Пручкина М.А., Приходько Ю.Н., Шнейдер Ю.А., Каримова Е.В.Zhivaeva T.S., Pruchkina M.A., Prikhodko Y.N., Shneyder Y.A., Karimova E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://phytosanitary.vniikr.ru/jour/article/view/160

Вирус мозаики пепино (Pepino mosaic virus, PepMV) – представитель рода Potexvirus, является карантинным объектом для стран ЕАЭС, ЕОКЗР и ряда стран различных континентов. Основные растения-хозяева вируса – томат и баклажан, но также он поражает перец, картофель и пепино. Потери урожая плодов томата в результате заражения PepMV могут достигать 30-40%. Вирус мозаики пепино характеризуется многообразием путей распространения и способен длительное время сохраняться в растительных остатках, почве, воде, растворах для гидропоники и на различных инертных поверхностях. Важное значение в распространении PepMV имеет семенная инфекция. С зараженными семенами вирус способен распространяться на новые территории, а единичные зараженные семена в партии способны в процессе вегетации нанести колоссальный ущерб производителям овощных культур. Мировая популяция вируса мозаики пепино состоит из пяти генетически различающихся штаммов, что затрудняет его диагностику с использованием молекулярных методов. Целью проводимых исследований являлся поиск праймеров, позволяющих осуществлять универсальное выявление изолятов всех штаммов вируса мозаики пепино и праймеров, позволяющих проводить высокоспецифичное определение штаммовой принадлежности выявляемых изолятов этого вируса. По результатам испытания 13 пар праймеров определены три пары праймеров для универсального выявления всех штаммов PepMV и две пары праймеров для высокоспецифичного определения штаммов Eu/Peruvian. Констатирована необходимость дальнейшего скрининга праймеров для высокоспецифичного определения штаммов CH1/US1 и СН2 PepMV.

Pepino mosaic virus (PepMV), a representative of the genus Potexvirus, is a quarantine pest for the EAEU and the EPPO countries, as well as some other countries on different continents. The main host plants of the virus are tomato and eggplant, though it also affects peppers, potatoes and pepino. Tomato fruit yield losses as a result of PepMV infection can reach 30-40%. PepMV is characterized by various pathways and can maintain in plant debris, soil, water, hydroponic solutions and on various inert surfaces for a long time. Seed infection plays an important role in the spread of PepMV. With infected seeds, the virus can spread to new territories, and single infected seeds in a batch can cause colossal damage to vegetable producers during the growing season. The global PepMV population consists of five genetically distinct strains, making it difficult to diagnose using molecular methods. The purpose of the research was to search for primers that allow universal detection of isolates of all PepMV strains and primers that allow highly specific determination of the strain affiliation of the detected isolates of this virus. Based on the results of testing 13 pairs of primers, three pairs of primers were identified for the universal detection of all PepMV strains and two pairs of primers for the highly specific detection of Eu/Peruvian strains. The need for further screening of primers for highly specific determination of CH1/US1 and CH2 PepMV strains has been stated.

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The authors declare that there are no conflicts of interest present.