Optimized Approach for the Detection of Heterodera spp. and the Quarantine Species Heterodera glycines
https://doi.org/10.69536/FKR.2020.96.92.001
Abstract
The approbation and optimization of PCR tests using Russian commercial reagents kits for the DNA extraction and amplification of the soybean cyst nematode Heterodera glycines Ichinohe in classic PCR format has been carried out. The suitability of Russian commercial kits for this study has been confirmed.
About the Authors
A. IvanovRussian Federation
Junior Researcher of Helminthology Laboratory Testing Laboratory Center,
Bykovo, Ramenskoye, Moscow region
S. Sudarikova
Russian Federation
Senior Researcher of Helminthology Laboratory Testing Laboratory Center
Bykovo, Ramenskoye, Moscow region
E. Khudyakova
Russian Federation
Head of Helminthology Laboratory Testing Laboratory Center
Bykovo, Ramenskoye, Moscow region
References
1. EPPO Global Database, 2020. URL: https://gd.eppo.int (last accessed: 01.09.2020).
2. Ichinohe M. On the soy bean nematode, Heterodera glycines n. sp., from Japan. Oyo-Dobutsugaku-Zasshi. 1952; 17 (1–2): 1–4.
3. Volkova T.V. Soybean nematode (Tylenchida: Heteroderidae: Heterodera glycines) in the Primorsky Krai [Soyevaya nematoda (Tylenchida: Heteroderidae: Heterodera glycines) v Primorskom Krae]. Vladivostok: Dalnauka, 2003. 91 pp. (In Russian.)
4. Qing Yu. Soybean Cyst Nematode (Heterodera glycines Ichinohe). In: Soybean Physiology and Biochemistry. Prof. Hany El-Shemy (Ed.). InTech, 2011. 488 p.
5. Common List of Plant Quarantine Objects of the Eurasian Economic Union approved by the Decision of the Council of the Eurasian Economic Commission as of 30.11.2016 № 158. As amended and supplemented from 03/30/2018 (changes from 05/02/2018 – Decision of the Council of the Eurasian Economic Commission from 03/30/2018, No. 25). 2018. (In Russian.)
6. The List of Quarantine Objects approved by order of the Ministry of Agriculture of the Russian Federation dated December 15, 2014, № 501. (In Russian.)
7. Butorina N.N., Zinovieva S.V., Kulinich O.A. et al. Applied Nematology [Prikladnaya nematologiya]. RAS Institute of Parasitology. Moscow: Nauka, 2006. 350 p. (In Russian.)
8. Syntol company official website. URL: https://www.syntol.ru (last accessed: 03.09.2020). (In Russian.)
9. Evrogen company official website. URL: http://evrogen.ru (last accessed: 03.09.2020). (In Russian.)
10. AgroDiagnostica company official website. URL: https://www.agrodiagnostica.ru (last accessed: 03.09.2020). (In Russian.)
11. Dialat Ltd. company official website. URL: http://dialat.ru (last accessed: 03.09.2020). (In Russian.)
12. Hu M., Hoglund J. Mutation scanning analysis of mitochondrial cytochrome c oxidase subunit 1 reveals limited gene flow among bovine lungworm subpopulations in Sweden. Electrophoresis. 2002; 23 (19): 3357–3363.
13. Subbotin S., Peng D., Moens M. A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Nematology. 2001; 3 (4): 365–371.
14. MR VNIIKR № 32-2015 “Methodological recommendations on the detection and identification of the soybean cyst nematode Heterodera glycines (Ichinohe)”. (In Russian.)
15. STO VNIIKR 6.001-2010 “Potato cyst nematodes Globodera rostochiensis (Woll.) Behrens and Globodera pallida (Stone) Behrens. Detection and identification methods”. (In Russian.)
16. Sudarikova S.V., Ivanov A.V., Khudiakova E.A. PCR Diagnostics to Enhance Detection of Globodera rostochiensis (Wollenweber) Skarbilovich [Sovershenstvovanie PCR-diagnostiki zolotistoy kartofelnoy tsistoobrazuyushey nematody Globodera rostochiensis (Wollenweber) Skarbilovich]. Plant Health. Research and Practice. 2019; 4 (30): 9–22. (In Russian.)
17. Basic Local Alignment Seаrch Tool. NCBI. URL: https://blast.ncbi.nlm.nih.gov/Blast.cgi (last accessed: 03.09.2020).
Review
For citations:
Ivanov A., Sudarikova S., Khudyakova E. Optimized Approach for the Detection of Heterodera spp. and the Quarantine Species Heterodera glycines. Plant Health and Quarantine. 2020;(4):30-39. https://doi.org/10.69536/FKR.2020.96.92.001