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Optimized Approach for the Detection of Heterodera spp. and the Quarantine Species Heterodera glycines

https://doi.org/10.69536/FKR.2020.96.92.001

Abstract

The approbation and optimization of PCR tests using Russian commercial reagents kits for the DNA extraction and amplification of the soybean cyst nematode Heterodera glycines Ichinohe in classic PCR format has been carried out. The suitability of Russian commercial kits for this study has been confirmed.

About the Authors

A. Ivanov
All-Russian Plant Quarantine Center
Russian Federation

Junior Researcher of Helminthology Laboratory Testing Laboratory Center,

Bykovo, Ramenskoye, Moscow region



S. Sudarikova
All-Russian Plant Quarantine Center
Russian Federation

Senior Researcher of Helminthology Laboratory Testing Laboratory Center

Bykovo, Ramenskoye, Moscow region



E. Khudyakova
All-Russian Plant Quarantine Center
Russian Federation

Head of Helminthology Laboratory Testing Laboratory Center

Bykovo, Ramenskoye, Moscow region



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Review

For citations:


Ivanov A., Sudarikova S., Khudyakova E. Optimized Approach for the Detection of Heterodera spp. and the Quarantine Species Heterodera glycines. Plant Health and Quarantine. 2020;(4):30-39. https://doi.org/10.69536/FKR.2020.96.92.001

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ISSN 2782-327X (Print)