Chrysanthemum stem necrosis virus: area, biology, diagnosis methods
https://doi.org/10.69536/FKR.2021.34.41.001
Abstract
Chrysanthemum stem necrosis virus (CSNV), common in South Korea, Brazil, Iran and Japan, with a strong infection causes the death of host plants, the main of which are Dendranthema x grandiflorum and tomato. Effective vectors of the virus are western flower thrips and tomato thrips. There is a real risk of introduction and massive adaptation of this virus both in green – houses and open ground in the southern regions of the Russian Federation, which accounts for the necessity of its reliable diagnosis by laboratory methods. FGBU “VNIIKR” has experimentally proved that the method of polymerase chain reaction in real time (RT-PCR) turned out to be a more sensitive method compared to classical PCR. Chrysanthemum stem necrosis virus was not detected in chrysanthemum plant samples from several greenhouse farms analyzed by PCR.
About the Authors
O. N. Kornilaeva
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Olga Kornilaeva – researcher, head of Virology Laboratory, Testing Laboratory Center.
Bykovo, Ramenskoye, Moscow Oblast
O. O. Beloshapkina
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Olga Beloshapkina – Doctor of Agriculture, professor of the Plant Protection Department.
Moscow
Yu. N. Prikhodko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuriy Prikhodko – PhD in Agriculture, leading researcher, Research and Methodology Departmet for Virology and Bacteriology
Bykovo, Ramenskoye, Moscow Oblast
References
1. Koshkin E., Panfilova O., Pilshchikova N. Quality of floriculture products: problems and solutions [Kachestvo produktsii tsvetovodstva: problemy i resheniya]. M.: RGAU-MSKHA, 2012; 16–19 (in Russian).
2. Morozova O., Schneider Yu., Prikhodko Yu., Beloshapkina O. Verification of diagnostic methods for impatiens necrotic spot virus and tomato spotted wilt virus in host plants and insect vectors [Otrabotka metodov diagnostiki virusov nekroticheskoy pyatnistosti balzamina i bronzovosti tomata v rasteniyakhkhozyayevakh i nasekomykh-perenoschikakh]. Plant Health. Research and Practice, 2017; 4 (22): 25–28 (in Russian).
3. Alexandre M., Duarte L., Rivas M., Chagas C. Mixed infection by Tospovirus species in ornamental crops in Sao Paulo state, Brazil. Summa phytopathol, 1999; 25: 353–356.
4. Bezerra I., de Resende R., Pozzer L., Nagata T., Kormelink R., de Ávila A.C. Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology, 1999; 89: 823–830.
5. Boben J., Mehle N., Pirc M., Mavric Plesko I., Ravnikar M. New molecular diagnostic methods for detection of Chrysanthemum stem necrosis virus (CSNV). Acta Biologica Slovenica, 2007; 50: 41–51.
6. Bucher E., Sijen T., de Haan P., Goldbach R., Prins M. Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. Journal of Virology, 2003; 77: 1329–1336.
7. De Jongle K., Morio S., Maes M. First outbreak of Chrysanthemum stem necrotic virus (CSNV) in Belgium. New Disease Reports, 2013; 28: 14–15.
8. De Oliveira A., Melo F., Inoue-Nagata A., Nagata T., Kitajima W., Resende R.O. Characterization of Bean necrotic mosaic virus: A member of a novel evolutionary lineage within the genus Tospovirus. Plos ONE, 2012; 7: 1–9.
9. Eiras M., Resende R., Missiaggia A., de Ávila A. RT-PCR and dot blot hybridization methods for a universal detection of tospoviruses. Fitopatologia Brasileira, 2001; 26: 170–175.
10. EPPO. Chrysanthemum stem necrosis tospovirus. Data sheets on quarantine pests. Bulletin OEPP/EPPO Bulletin, 2005; 35: 409–412.
11. Jafarpour B., Sabokkhiz M.A., Rastegar M. First report of CSNV in Iran and occurrence of some viral diseases of ornamental plants in Mashhad region, Iran. Petria, 2010; 20: 67–68.
12. King A., Adams M., Carstens E.B, Lefkowitz E. Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses. International Union of Microbiological Societies Vd. Elsevier Academic Press. 2012, London, UK. 1327 p.
13. Logemann J., Schell J., Willmitzer L. Improved method for the isolation of RNA from plant tissues. Analytical Biochemistry, 1987; 163: 16–20.
14. Mumford R., Barker I., Wood K. An improved method for the detection of Tospoviruses using the polymerase chain reaction. Journal of Virological Methods, 1996; 57: 109–115.
15. Okuda M., Hanada K. RT-PCR for detecting five distinct Tospovirus species using degenerate primers and dsRNA template. J. Virol. Methods, 2001; 96: 149–156.
16. Takeshita M., Nagai N., Okuda M., Matsuura S., Okuda S., Furuya N., Tsuchiya K. Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan. Eur. J. Plant Pathol, 2011; 131: 9–14.
17. Verhoeven J., Roenhorst J., Cotes I., Peters D. Detection of a novel tospovirus in chrysanthemum. Acta Horticulturae, 1996; 432: 44–51.
18. Wu P., Chien W., Okuda M., Takeshita M., Yeh S., Wang Y., Chen T. Genetic and serological characterization of Chrysanthemum stem necrosis virus, a member of the genus Tospovirus. Arch. Virology, 2014; 27: 634–643.
19. EPPO, 2021. EPPO Global Database. URL: https://gd.eppo.int (last accessed: 12.04.2021).
Review
For citations:
Kornilaeva O.N., Beloshapkina O.O., Prikhodko Yu.N. Chrysanthemum stem necrosis virus: area, biology, diagnosis methods. Plant Health and Quarantine. 2021;(4):16-23. https://doi.org/10.69536/FKR.2021.34.41.001
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