Using a pathogenicity test for the diagnosis of the bacterial pea blight agent
https://doi.org/10.69536/FKR.2021.83.15.001
Abstract
The agent of the bacterial pea blight, Pseudomonas syringae pv. pisi (Sackett) Young et al. (P. s. pv. pisi), is a main phytopathogen of pea (Pisum sativum L.). National Plant Protection Organizations (NPPOs) require reliable techniques to confirm the absence of the bacterial blight agent. The aim of this work is to study the possibility of applying a pathogenicity test for the diagnosis of the bacterial pea blight agent. During the approbation of the test, we used pea plants of the Pioner variety, strain P. s. pv. pisi CFBP 2105, “Proba-GS” kit produced by LLC “AgroDiagnostika” (Russia), and the primers AN7F/AN7R (Qing Ch. et al., 2016). After preliminary tests, the proposed test for pathogenicity can be used to determine the virulence of the isolate, and can also be proposed as one of the additional confirmatory methods for identifying a phytopathogen in a test sample when developing guidelines for detecting and identifying the causative agent of the bacterial pea blight.
About the Authors
I. M. Ignatyeva
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Irina Ignatyeva - Researcher, Bacteriology and GMO Analysis Laboratory, Laboratory Testing Center, FGBU VNIIKR.
Bykovo, Ramenskoye, Moscow Oblast
O. Yu. Slovareva
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR)
Russian Federation
Russian Federation
Olga Slovareva - Junior Researcher, Bacteriology and GMO Analysis Laboratory, Laboratory Testing Center, FGBU VNIIKR.
Bykovo, Ramenskoye, Moscow Oblast
I. G. Bashkirova
FGBU All-Russian Plant Quarantine Center (FGBU VNIIKR); FGAOU VO Peoples’ Friendship University of Russia (FGAOU VO RUDN)
Russian Federation
Russian Federation
Ida Bashkirova - Junior Researcher, Bacteriology and GMO Analysis Laboratory, Laboratory Testing
Center, FGBU VNIIKR; Post-graduate student of the Federal State Autonomous Educational Institution of Higher Education RUDN.
Bykovo, Ramenskoye, Moscow Oblast; Moscow
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Review
For citations:
Ignatyeva I.M., Slovareva O.Yu., Bashkirova I.G. Using a pathogenicity test for the diagnosis of the bacterial pea blight agent. Plant Health and Quarantine. 2021;(3):40-46. https://doi.org/10.69536/FKR.2021.83.15.001
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