Study of the genetic peculiarities of the causative agent of stem canker of sunflower
https://doi.org/10.69536/FKR.2021.42.76.001
Abstract
Stem canker of sunflower is a harmful disease in the countries of industrial cultivation of seed and commercial sunflowers. In the last decade, new species of Diaporthe genus have been described on the sunflower, which have little differences in morphological characteristics. Therefore, it is necessary to develop molecular genetic methods that would allow to conduct research in short terms and provide high accuracy of the pathogen identification. During the study, the data on the pathogen and molecular genetic diagnosis methods available in international practice have been collected and analyzed. A list of species of Diaporthe genus registered on the sunflower has been made. The most variable regions of the genome have been identified.
About the Authors
T. A. Surina
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Tatyana Surina, PhD in Biology, head and senior researcher of Research Department for Molecular Genetic Methods of Diagnosis, 140150, 32 Pogranichnaya St., Bykovo, Ramenskoye, Moscow Oblast, Russia
O. V. Skripka
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Olga Skripka, PhD in Biology, senior researcher of Research and Methodology Department for Mycology and Helminthology, Bykovo, Ramenskoye, Moscow Oblast, Russia
E. R. Ruchkov
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Egor Ruchkov, junior researcher of Research and Methodology Department for Mycology and Helminthology, Bykovo, Ramenskoye, Moscow Oblast, Russia
References
1. Masirevic S., Gulya T.J. Sclerotinia and Phomopsis – two devastating sunflower pathogens // Field Crops Research. 1992; 30: 271–300.
2. Thompson S.M., Tan Y.P., Young A.J., Neate S.M., Aitken E.A.B., Shivas R.G. Stem cankers on sunflower (Helianthus annuus) in Australia reveal a complex of pathogenic Diaporthe (Phomopsis) species // Persoonia. 2011; 27: 80–89.
3. Dolzhenko E.G. Biology of the fungus Phomopsis helianthi and its control measures in Krasnodar Krai [Biologiya griba Phomopsis helianthi i mery bor’by s nim v usloviyakh Krasnodarskogo kraya]. Author’s abstract for the degree of PhD in Biology. Krasnodar, Kuban State Agrarian University, 2000. 25 pp.
4. Piven V.T., Alifirova T.P., Shulyak I.I., Murasadilova N.V., Saenko G.M. The sunflower seeds as the source of preservation and spreading of phomopsis [Semena podsolnechnika – istochnik sokhraneniya i rasprostraneniya fomopsisa]. Plant protection and quarantine. 2010; 1: 36–40 (in Russian).
5. Gomzhina M.M., Gannibal F.B. The first detection of the fungus Diaporthe phaseolorum on sunflower in Russia [Pervaya nakhodka griba Diaporthe phaseolorum na podsolnechnike v Rossii]. Microbilogy Research Independent Journal. 2018; 5–1: 59–64 (in Russian).
6. Carbone I., Kohn L.M. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia. 1999; 91–3: 553–556.
7. Glass N.L., Donaldson G.C. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 1995; 61–4: 1323–1330.
8. O’Donnell K. еt al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences. 1998; 95–5: 2044–2049.
9. White T.J., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications. 1990; 18: 315–322.
Review
For citations:
Surina T.A., Skripka O.V., Ruchkov E.R. Study of the genetic peculiarities of the causative agent of stem canker of sunflower. Plant Health and Quarantine. 2021;(1):42-48. https://doi.org/10.69536/FKR.2021.42.76.001
Views:
455
Email this article 