Application of polymerase chain reaction method for identification of preimaginal stages of Drosophila suzukii (Matsumura, 1931)
https://doi.org/10.69536/FKR.2020.50.28.001
Abstract
In the process of work, the possibility of application of polymerase chain reaction (PCR) of certain COI gene fragments for identification of preimaginal developmental stages of spotted-wing drosophila Drosophila suzukii and possibility of application of these methods for laboratory diagnostics were studied. The article presents original data (base sequences) for identification of the target object by PCR method as well as further use for selection of species-specific genetic markers.
About the Authors
E. V. Kolesnikova
FGBU “VNIIKR”
Russian Federation
Russian Federation
Head of the Entomology Laboratory of the Laboratory Testing Center
G. N. Bondarenko
FGBU “VNIIKR”; Senior Lecturer of the Agrarian and Technological Institute
Russian Federation
Russian Federation
PhD in Biology, Senior Researcher, Head of the Laboratory Testing Center; FGAOU VO “Peoples’ Friendship University of Russia” (RUDN University)
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Review
For citations:
Kolesnikova E.V., Bondarenko G.N. Application of polymerase chain reaction method for identification of preimaginal stages of Drosophila suzukii (Matsumura, 1931). Plant Health and Quarantine. 2020;(3):32-40. https://doi.org/10.69536/FKR.2020.50.28.001
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