Diagnosis of begomoviruses by PCR
https://doi.org/10.69536/FKR.2020.91.31.001
Abstract
The genus Begomovirus includes more than 190 species of viruses, which are harmful pathogens of vegetable and field crops. Tomato yellow leaf curl virus (TYLCV), which is widely spread in Europe, is a quarantine object for the Russian Federation, EAEU and EPPO. Identifying begomoviruses to the species level involves certain difficulties due to the high identity of their genome. The article presents the results of testing 19 pairs of universal and specific primers for the diagnosis of begomoviruses: tomato leaf curl virus (TYLCV), tomato yellow leaf curl Sardinia virus (TYLCSV), and tomato leaf curl New Delhi virus (ToLCNDV) by conventional and real-time PCR methods.
About the Authors
E. N. Lozovaia
FGBU “VNIIKR”
Russian Federation
Russian Federation
Researcher of the Postgraduate Study Department
Y. N. Prikhodko
FGBU “VNIIKR”
Russian Federation
Russian Federation
PhD in Agriculture, Leading Researcher of the Research and Methodology Department for Virology and Bacteriology
T. S. Zhivaeva
FGBU “VNIIKR”
Russian Federation
Russian Federation
Researcher of the Research and Methodology Department for Virology and Bacteriology
Y. A. Shneyder
FGBU “VNIIKR”
Russian Federation
Russian Federation
PhD in Biology, Acting Deputy Director
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Review
For citations:
Lozovaia E.N., Prikhodko Y.N., Zhivaeva T.S., Shneyder Y.A. Diagnosis of begomoviruses by PCR. Plant Health and Quarantine. 2020;1(2):41-54. https://doi.org/10.69536/FKR.2020.91.31.001
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