Development and validation of methods for diagnosing Wheat dwarf virus (WDV) (Mastrevirus hordei)
https://doi.org/10.69536/FKR.2026.50.73.004
Abstract
Wheat dwarf virus (WDV), or Mastrevirus hordei, is a harmful pathogen of wheat, barley, rye, triticale, oats, and numerous species of perennial cereal grasses. Wheat yield losses due to infection by this virus can reach 100%. WDV is transmitted by Psammotettix striatus L. Widely spread in the Russian Federation.
To develop guidelines for the detection and identification of WDV, a test system for ELISA by Loewe Biochemica GmbH (Germany), two test systems for real-time PCR and 7 pairs of primers for classical PCR were tested.
The WDV ELISA test system tested was found to be characterized by a sufficiently high specificity for the target and can be recommended for screening tests for this virus. A similar conclusion was reached regarding the RTIC-F/RTIC-R/RTIC-Probe primers and probe (Wang et al., 2016), which enable the diagnosis of WDVB and WDV-W isolates with high specificity, sensitivity, and reproducibility. It was established that the WDV40F/WDV-806R and WDV-WFb/WDV-WRb primers enable the diagnosis of isolates of the WDV-W and WDV-B strains, while the WDV-F/WDV-R, WDV-TF/WDV-T-R and WDV-1F/WDV-WRb primers are characterized by specificity to the WDV-W strain, and the WDV-H-F/WDV-H-R and WDV-WFb/WDV-4R primers are specific to the WDV-B strain. To conduct confirmatory tests for the presence of WDV, classical PCR protocols with the WDV-WFb/WDV-WRb and WDV-F/WDV-R primers have been developed, ensuring high exclusivity, inclusiveness and sensitivity of tests.
About the Authors
E. N. Lozovaya
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Evgenia Lozovaya, Researcher, Postgraduate Department
Bykovo, Ramenskoye, Moscow Oblast, 140150
Y. N. Prikhodko
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuri Prikhodko, PhD in Agriculture, Leading Researcher, Research and Methodology Department of Virology
Bykovo, Ramenskoye, Moscow Oblast, 140150
T. S. Zhivaeva
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Tatiana Zhivaeva, Researcher, Research and Methodology Department of Virology
Bykovo, Ramenskoye, Moscow Oblast, 140150
I. G. Bashkirova
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Ida Bashkirova, PhD in Biology, Researcher, Research and Methodology Department of Virology
Bykovo, Ramenskoye, Moscow Oblast, 140150
A. A. Emelyanova
All-Russian Plant Quarantine Center (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Anastasia Emelyanova, Junior Researcher, Research and Methodology Department of Virology
Bykovo, Ramenskoye, Moscow Oblast, 140150
Y. A. Shneyder
All-Russian Plant Quarantine Center (FGBU “VNIIKR”);
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Yuri Shneyder, PhD in Biology, Head of Research and Methodology Department of Virology, Leading Researcher; Associate Professor of the Plant Protection Department
Bykovo, Ramenskoye, Moscow Oblast, 140150
Moscow, 127434
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Review
For citations:
Lozovaya E.N., Prikhodko Y.N., Zhivaeva T.S., Bashkirova I.G., Emelyanova A.A., Shneyder Y.A. Development and validation of methods for diagnosing Wheat dwarf virus (WDV) (Mastrevirus hordei). Plant Health and Quarantine. 2026;(1):38-56. (In Russ.) https://doi.org/10.69536/FKR.2026.50.73.004
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