Conventional and modern methods for the diagnosis of Colletotrichum fungi on garden strawberry Fragaria ananassa
https://doi.org/10.69536/FKR.2020.64.66.001
Abstract
The article overviews information on the species of the genus Colletotrichum that affect strawberry plants, including the Colletotrichum acutatum and the Colletotrichum gloeosporioides species complexes. It also specifies difficulties associated with the direct identification of pathogens and describes features of mycological research diagnosis and application of the complex approach using conventional and modern molecular genetic methods for precise in vitro analysis.
About the Authors
Y. V. Tsvetkova
FGBU “VNIIKR”
Russian Federation
Russian Federation
Agronomist of the Mycology Laboratory of the LTC
A. A. Kuznetsova
FGBU “VNIIKR”
Russian Federation
Russian Federation
Head of the Mycology Laboratory of the LTC, Senior Researcher
References
1. Bondarenko G.N., Belkin D.L. et al. Methodological recommendations for sequencing in the diagnosis of quarantine objects and other organisms [Metodicheskie rekomendacii po provedeniyu sekvenirovaniya pri diagnostike karantinnyh ob”ektov i drugih organizmov]. M.: FGBU “VNIIKR”, 2018: 37 pp. (in Russian).
2. Kotova V.V., Kungurtseva O.V. Anthracnose of agricultural plants [Antraknoz sel’skohozyajstvennyh rastenij]. Prilozhenie k zhurnalu Vestnik zashchity rastenij. SPb.: VIZR, 2014; 11: 132 pp. (in Russian).
3. Petina V.V., Skripka O.V. et al. Methodological recommendations for the detection and identification of strawberry anthracnose pathogen Colletotrichum acutatum [Metodicheskie rekomendacii po vyyavleniyu i identifikacii vozbuditelya antraknoza zemlyaniki Colletotrichum acutatum J.H. Simmonds]. M.: FGBU “VNIIKR”, 2013 (in Russian).
4. Damm U., Baroncelli R., Cai L. et al. Colletotrichum: species, ecology and interactions. IMA Fungus. 2010; 1: 161-165.
5. Damm U., Cannon P.F., Woudenberg J.H.C., Crous P.W. The Colletotrichum acutatum species complex. Studies in Mycology. 2012; 73: 37-113. DOI: 10.3114/sim0010.
6. Peres N.A., Timmer L.W., Adaskaveg J.E., Correll J.C. Life styles of Colletotrichum acutatum. Plant Disease. 2005; 89 (8): 784-796. DOI: 10.1094/PD-89-0784.
7. Sreenivasaprasad S., Sharada K., Brown A.E., Mills P.R. PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathology. 1996; 45: 650-655.
8. Weir B.S., Johnston P.R., Damm U. The Colletotrichum gloeosporioides species complex. Studies in Mycology. 2012; 73: 115-180. DOI: 10.3114/sim0011.
9. White T.J., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, eds. Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., eds. PCR Protocols: A Guide to Methods and Amplifications. San Diego, CA, USA: Academic Press, 1990: 315-322.
10. The National Center for Biotechnology. Colletotrichum nymphaeae culture-collection CBS: 125959. URL: https://www.ncbi.nlm.nih.gov/nuccore/ JQ948246.1.
Review
For citations:
Tsvetkova Y.V., Kuznetsova A.A. Conventional and modern methods for the diagnosis of Colletotrichum fungi on garden strawberry Fragaria ananassa. Plant Health and Quarantine. 2020;1(1):34-42. https://doi.org/10.69536/FKR.2020.64.66.001
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