Detection of Potato mop-top virus by real-time RT-PCR
https://doi.org/10.69536/x7031-3409-6918-k
Abstract
Potato (Solanum tuberosum) is the most important agricultural crop. Currently, potatoes are cultivated on more than 18 million hectares in the world. The object of study of the presented article is Potato mop-top virus (PMTV), a serious pathogen causing damage to potato all over the world. This pest reduces the yield, causes characteristic damage to the pulp and tuber surface, which affects the commercial quality and the possibility of using them for processing. Climatic conditions in the countries where the virus is spreading are similar to those for potato cultivation in the Russian Federation. Besides, its vector, fungus Spongospora subterranea f. sp. subterranea, causing powdery scab of potato, is widespread in Russia. There are no reliable methods to control PMTV, so the virus can also adapt and spread throughout Russia. In this regard, it is important to have effective methods for detecting and identifying this organism.
The study compared a commercial real-time polymerase chain reaction (real-time PCR) kit with species-specific primers for the identification of PMTV. The applicability of commercial reverse transcription kits was also evaluated. The most successful combinations of reagents for cDNA synthesis, primers, and amplification modes for the detection of PMTV were tested on potato samples taken in the Irkutsk Oblast, Volgograd Oblast and Rostov Oblast, as well as Primorsky Krai.
Keywords
PMTV,
potato phytopathogens,
molecular diagnostics,
reverse transcription,
phytosanitary safety,
Spongospora subterranea
Supporting agencies: The authors express their gratitude to the staff of the Research and Methodology Department of Virology and Bacteriology, FGBU “VNIIKR”, for their help in conducting research, as well as to the junior researcher of the Primorsky Branch of FGBU “VNIIKR” N.A. Khorina for providing a photo of tubers with PMTV symptoms.
About the Authors
M. A. Pruchkina
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Maria Pruchkina, PhD student, Agronomist of Phytosanitary Risks and International Cooperation Department
Bykovo, Urban district Ramensky, Moscow Oblast, 140150
Yu. A. Shneyder
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuri Shneyder, PhD in Biology, Head of Research and Methodology and Experimental Center, Leading Researcher
Bykovo, Urban district Ramensky, Moscow Oblast, 140150
T. S. Zhivaeva
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Tatiana Zhivaeva, Researcher, Research and Methodology Department of Virology and Bacteriology
Bykovo, Urban district Ramensky, Moscow Oblast, 140150
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For citations:
Pruchkina M.A., Shneyder Yu.A., Zhivaeva T.S. Detection of Potato mop-top virus by real-time RT-PCR. Plant Health and Quarantine. 2023;(3):25-31. https://doi.org/10.69536/x7031-3409-6918-k
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