Testing a test system for the diagnosis of Xanthomonas euvesicatoria pv. euvesicatoria
https://doi.org/10.69536/a6147-0848-4962-i
Abstract
Bacterial spot of tomato is worldwide spread and causes significant economic damage in tomato and pepper cultivation. Diagnosis is complicated by the fact that the disease is caused by four types of bacteria of the genus Xanthomonas. Seeds are the main pathway for bacteriosis over long distances. The European and Mediterranean Plant Protection Organization (EPPO) has included a group of causative agents of black bacterial spot in List A2 of the List of Quarantine Objects. Detection and identification of phytopathogens at all stages of tomato and pepper cultivation will allow taking timely measures to protect plants, which will significantly reduce crop losses and increase the economic efficiency of domestic vegetable production. Existing schemes for diagnosing the disease imply large labor costs and a long period of research, which is unacceptable in routine laboratory diagnosis. The article presents the results of studies on the diagnosis of one of the pathogens of black bacterial spot (X. euvesicatoria pv. euvesicatoria). To establish the degree of influence of the plant matrix on the final results of the samples study, we conducted a series of experiments on artificial infection with X. euvesicatoria pv. euvesicatoria of extracts of tomato vegetative parts and seeds. In the course of testing the sample preparation technique with further DNA isolation and PCR analysis of artificially infected samples, it was found that PCR inhibition does not occur. Analytical sensitivity of the diagnostic test system for X. euvesicatoria pv. euvesicatoria produced by AgroDiagnostics was 103 cfu/ml. The studies were carried out on the basis of FGBU “VNIIKR”.
About the Authors
I. N. Pisareva
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Irina Pisareva, Researcher, Research and Methodology Department of Virology and Bacteriology
Bykovo, Ramenskoye, Moscow Oblast
A. B. Yaremko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Anastasia Yaremko, Junior Researcher, Research Department of Molecular and Genetic Diagnosis Methods
Bykovo, Ramenskoye, Moscow Oblast
S. I. Prikhodko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Svetlana Prikhodko, Researcher, Head of Laboratory of Bacteriology and GMO Analysis, Testing Laboratory Center
Bykovo, Ramenskoye, Moscow Oblast
E. YU. Shneyder
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Elena Shneyder, Senior Researcher, Research and Methodology Department of Virology and Bacteriology
Bykovo, Ramenskoye, Moscow Oblast
References
1. Akhatov A. The world of tomato through the eyes of a phytopathologist [Mir tomata glazami fitopatologa]. 3rd ed., rev. and additional. M.: Tov-in scientific. editions of KMK, 2016, 292 p. (in Russian).
2. Beloshapkina O. et al. Plant protection: phytopathology and entomology [Zashchita rasteniy: fitopatologiya i entomologiya]. Rostov n/a: Phoenix, 2017, 478 p. (in Russian).
3. Ivantsova E. Tomato diseases in the Lower Volga region [Bolezni tomata v Nizhnevolzhskom regione]. Farmer. Volga Region, 2017; 2: 80–82 (in Russian).
4. Netrusov A. et al. Workshop on microbiology [Praktikum po mikrobiologii]. M.: Academy, 2005, 571 p. (in Russian).
5. Shneyder E., Shneyder Yu. Systematization of the list and categorization of pests associated with the import, export and movement of seeds of Solanaceae crops [Sistematizatsiya perechnya i kategorizatsiya vrednykh organizmov, svyazannykh s importirovaniyem, eksportirovaniyem i peremeshcheniyem semyan paslenovykh kultur]. Research, FGBU “VNIIKR”, 2020, 147 p.
6. EPPO. PM 7/110 (1) Xanthomonas spp. (Xanthomonas euvesicatoria, Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper. Bulletin OEPP/EPPO Bulletin, 2013; 43 (1): 7–20.
7. Fatmi M., Walcott R., Schaad N. Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material, Second Edition. Minnesota: The American Phytopathological Society (APS), 2017, 360 p.
8. ISF, 2017. Method for the detection of Xanthomonas spp. in Tomato seed. URL: https://worldseed.org/wp-content/uploads/2022/01/2017_Protocol_Tomato_Xanthomonas_spp_v5.pdf (last accessed: 30.06.2022).
9. Potnis N., Timilsina S., Strayer A., Shantharaj D., Barak J., Paret M., Vallad G., Jones J. Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge. Molecular Plant Pathology, 2015; 16 (9): 907–920.
10. Roach R., Mann R., Gambley C., Shivas R., Rodoni B. Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia. European Journal of Plant Pathology, 2018; 150 (3): 595–608.
11. Analysis of the tomato market in Russia in 2016–2020. Coronavirus impact assessment and forecast for 2021–2025 [Electronic resource]. URL: https://businesstat.ru/catalog/id8248 (last accessed: 30.09.2021).
12. CABI. Invasive Species Compendium. URL: https://www.cabi.org/isc (last accessed: 30.06.2022).
13. EPPO Global Database. URL: https://gd.eppo.int (last accessed: 30.06.2022).
Review
For citations:
Pisareva I.N., Yaremko A.B., Prikhodko S.I., Shneyder E.Yu. Testing a test system for the diagnosis of Xanthomonas euvesicatoria pv. euvesicatoria. Plant Health and Quarantine. 2022;(3):37-44. https://doi.org/10.69536/a6147-0848-4962-i
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