Using PCR Systems for the Diagnosis of Potato Blackleg Agents
https://doi.org/10.69536/FKR.2020.42.64.001
Abstract
Blackleg of potato is a bacteriosis widespread in the Russian Federation caused by pectolytic bacteria of the genus Pectobacterium, Enterobacteriaceae family. In 2009, Dickeya spp. agents of potato blackleg were identified in Russia: Dickeya dianthicola and Dickeya solani. The annual yield shortfall due to this disease can reach from 1–2 to 50–70%. The main pathway for the pathogen bacteria is connected to seed material movement. The detection and identification of plant pathogens causing potato blackleg require the design and optimization of highly sensitive laboratory methods for practical use. In 2019, within the framework of the Federal Scientific and Technical Program (FSTP) of agriculture development for 2017–2025, the RAS Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry and A.G. Lorkh Russian Potato Federal Research Center designed new test systems in real-time PCR (RT-PCR) for the diagnosis of bacteria causing potato blackleg, with analytical sensitivity for detecting the pathogen DNA at the level of 10–50 copies of plasmids, containing a specific PCR product, per reaction. The article presents the results of comparative testing of the new test systems for the diagnosis of the bacteria Pectobacterium atrosepticum, Dickeya dianthicola and Dickeya solani. When testing 20 varieties of seed potatoes delivered to the Testing Laboratory of A.G. Lorkh Russian Potato Federal Research Center by manufacturers from 4 regions of the Russian Federation, in 15 cases, complete coincidence of the analysis results was shown when using routine and new test systems.
About the Authors
A. Uskov
A.G. Lorkh Russian Potato Federal Research Center
Russian Federation
Russian Federation
Doctor of Agriculture, Leading Researcher and Head of Department
Kraskovo, Lyubertsy, Moscow region
I. Shmyglya
A.G. Lorkh Russian Potato Federal Research Center
Russian Federation
Russian Federation
Senior Researcher
Kraskovo, Lyubertsy, Moscow region
A. Stakheev
RAS Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
PhD in Biology, Researcher
Moscow
S. Zavriyev
RAS Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
Doctor of Biology, Corresponding Member of Russian Academy of Sciences, Head of Department
Moscow
Yu. Varitsev
A.G. Lorkh Russian Potato Federal Research Center
Russian Federation
Russian Federation
PhD in Biology, Leading Researcher
Kraskovo, Lyubertsy, Moscow region
P. Galushka
A.G. Lorkh Russian Potato Federal Research Center
Russian Federation
Russian Federation
PhD in Agriculture, Senior Researcher
Kraskovo, Lyubertsy, Moscow region
N. Suslova
A.G. Lorkh Russian Potato Federal Research Center
Russian Federation
Russian Federation
Junior Researcher
Kraskovo, Lyubertsy, Moscow region
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Review
For citations:
Uskov A., Shmyglya I., Stakheev A., Zavriyev S., Varitsev Yu., Galushka P., Suslova N. Using PCR Systems for the Diagnosis of Potato Blackleg Agents. Plant Health and Quarantine. 2020;(4):65-75. https://doi.org/10.69536/FKR.2020.42.64.001
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