Morphological identification methods of Bidens pilosa L. (Asteraceae) fruits: development and applicability testing
https://doi.org/10.69536/y3165-9744-5936-l
Abstract
Bidens pilosa L. (Asteraceae) is a weed and a quarantine object absent in the territory of the Eurasian Economic Union. The entry of invasive plants often occurs with imported cargo at the stage of dormant diaspores, represented by fruits for this plant. To prevent the entry, laboratory research is used to identify the fruits of the quarantine object in cargo batches. Identification of Bidens pilosa fruits during laboratory research is handicapped by the presence of similar non-quarantine weed species with a wide range, such as B. subalternans DC. and B. parviflora Willd., as well as the possibility of incorrect identification of the close quarantine species B. bipinnata L. Additional complexity is created by the lack of international, regional or national identification methods. In 2015, FGBU “VNIIKR” developed a methodic document for identifying Bidens pilosa fruits, but it has not yet been tested on a representative sample accumulated as a result of expeditionary research. Within this study, the applicability of the existing methodology was evaluated, and the possibility of identifying Bidens pilosa fruits by other characteristics was considered in a sample of 431 fruits from habitats with a wide geographical representation, including fruits of similar species. The accuracy of the existing identification method on the examined sample was determined to be 69.4%. Other identification methods based on 12 morphological fruit characteristics and their combinations were studied. A method providing identification of Bidens pilosa fruits with sufficient laboratory accuracy (not less than 95%) was not identified. Recommendations are given for expressing uncertainty in the existing method in testing laboratories
About the Authors
V. Kulakov
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Vitaly Kulakov, Senior Researcher – Head of Interlaboratory Comparisions Department
Bykovo, Urban district Ramensky, Moscow Oblast, 140150
S. Potanina
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Sofya Potanina, Junior Researcher, Research and Methodology Department of Invasive Plant Species
Bykovo, Urban district Ramensky, Moscow Oblast, 140150
Y. Kulakova
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuliana Kulakova, PhD in Biology, Leading Researcher – Head of Research and Methodology Department of Invasive Plant Species
Bykovo, Urban district Ramensky, Moscow Oblast, 140150
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Review
For citations:
Kulakov V., Potanina S., Kulakova Y. Morphological identification methods of Bidens pilosa L. (Asteraceae) fruits: development and applicability testing. Plant Health and Quarantine. 2024;(1):41-53. https://doi.org/10.69536/y3165-9744-5936-l
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