Study of pea cultivars resistance to pea bacterial blight Pseudomonas syringae pv. pisi
https://doi.org/10.69536/j1448-5952-6569-x
Abstract
Bacterial blight of pea is a main pea phytopathogen and is spread through seeds. In some countries, including those importing peas from Russia, Pseudomonas syringae pv. pisi causing bacteriosis of some leguminous crops is included in quarantine lists. Introducing detection and identification methods of the pathogen and determining resistant cultivars have become important in strategies for maintaining the level of pea exports from Russia. For this purpose, during this work, pea cultivars were selected and tested for their resistance to the phytopathogen. Five pea cultivars were planted for research. After germination occurred in the 2–3 leaf phase, 10 plants of each cultivar were inoculated with a bacterial suspension at a concentration of 106 CFU/ml. One plant was left as a negative control using sterile distilled water for inoculation. During the development of seedlings, infected plants were compared with a control sample, and the manifestation of disease symptoms was recorded. On the 22nd day after inoculation, samples of vegetative parts of the plants were taken and the presence of pathogen cells was monitored using PCR with primers AN7F/AN7R. According to the PCR results, in all plant samples (including asymptomatic ones), the presence of an amplification product with a size of 272 base pairs specific to the bacterial blight of peas pathogen was confirmed. As a result of the study, the susceptibility of pea cultivars to bacteriosis was revealed in the pea cultivars of Varyag, Altaisky Usatiy, Astronavt. The study determined that the most suitable cultivar for breeding, resistant to Pseudomonas syringae pv. pisi, was Ambrosia. The necessity of evaluating the development of phytopathogen infection symptoms together with molecular genetic methods for identifying bacterial DNA in plant cells to confirm the resistance of a cultivar to pea bacterial blight was also confirmed.
About the Authors
I. M. IgnatievaRussian Federation
Irina Ignatieva, Researcher, Head of Bacteriology and GMO Analysis Laboratory,
140150, Bykovo, Urban district Ramensky, Moscow Oblast.
A. S. Bakaeva
Russian Federation
Aleksandra Bakaeva, Agronomist, Bacteriology and GMO Analysis Laboratory,
140150, Bykovo, Urban district Ramensky, Moscow Oblast.
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Review
For citations:
Ignatieva I.M., Bakaeva A.S. Study of pea cultivars resistance to pea bacterial blight Pseudomonas syringae pv. pisi. Plant Health and Quarantine. 2023;(4):17-27. https://doi.org/10.69536/j1448-5952-6569-x