Approbation of the PCR method for species identification of Aphelenchoides besseyi Christie, 1942
https://doi.org/10.69536/h1279-3120-4564-k
Abstract
The article is dedicated to Aphelenchoides besseyi Christie, 1942 – rice leaf nematode. It is widespread in many rice-growing countries. Currently, it is excluded from the Common List of Quarantine Objects of the Eurasian Economic Union, however, it is regulated by a number of countries importing Russian products.
The pest is polyphagous and infects a wide range of plants. In the European and Mediterranean Plant Protection Organization (EPPO) countries, rice and strawberries are the main hosts most at risk from A. besseyi infestation (PM 7/39 (1), 2004). Since rice is an important food product, it occupies the second place (after wheat) in world agriculture in terms of sown area and gross grain harvest.
The article summarizes and analyzes scientific information on the geographical distribution, harmfulness of the rice leaf nematode A. besseyi, its bioecological features, and the method of morphological diagnosis. The methods of molecular genetic identification of the rice leaf nematode A. besseyi used in foreign research papers have been studied. When conducting our research, we used nematodes collected during expeditions to the Far East (Primorsky Krai, Khankaisky District) and Krasnodar Krai of the Russian Federation. The species identification of nematodes by morphological characteristics was carried out in accordance with the guidelines for the detection and identification of the rice nematode Aphelenchoides besseyi Christie (MR VNIIKR No. 89-2016). For laboratory research, both morphological and molecular methods are used. The development and application of modern diagnosis methods in the field of plant protection are relevant for the detection and identification of the rice leaf nematode A. besseyi.
The study was conducted with polymerase chain reaction (PCR) using commercial amplification mixtures 5x MasDDTaq MIX-2025 and 5x MasORTaq MIX- 2025 Dialat Ltd. (Russia), as well as 5х ScreenMix,5х ScreenMix-HS and 5х ScreenMix-HS (UDG) Evrogen (Russia).
About the Authors
A. V. Ivanov
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Junior Researcher, Helminthology Laboratory of Testing Laboratory Center
M. V. Ushkova
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Junior Researcher, Entomology Laboratory of Testing Laboratory Center
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Review
For citations:
Ivanov A.V., Ushkova M.V. Approbation of the PCR method for species identification of Aphelenchoides besseyi Christie, 1942. Plant Health and Quarantine. 2022;(4):46-56. (In Russ.) https://doi.org/10.69536/h1279-3120-4564-k
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