Analysis of genetic differences of some Pseudomonas syringae pathovars
https://doi.org/10.69536/s9564-4378-7598-q
Abstract
Pseudomonas syringae pv. maculicola is a phytopathogen affecting cruciferous plants worldwide. P. s. pv. maculicola is regulated by the phytosanitary requirements of the following countries: China, Israel, Mexico, Egypt, Sudan and Indonesia. Thus, there was a need to establish the compliance of the places of production and export products with the requirements of the countries, Russia’s trading partners, using laboratory diagnostics methods. Due to the high genetic similarity of P. syringae pathovars, when conducting laboratory diagnostics, of particular interest is the need to distinguish maculicola pathovar from tomato pathovar. These bacteria can simultaneously be present on plants of the Cruciferous (Cabbage) family and cause bacteriosis. It is also difficult to identify the causative agent of bacteriosis on cruciferous plants, the bacterium P. cannabina pv. alisalensis. This species has a wide range of host plants, including cruciferous plants. In addition, host plants areas and symptoms of P. s. pv. maculicola and P. с. pv. alisalensis coincide, which can also lead to confusion in the identification. In order to analyze the genetic differences of some Pseudomonas syringae pathovars, as well as the search for specific genetic markers that can be used in laboratory diagnosis of P. s. pv. maculicola, we searched for a potential target by studying proteins corresponding to 293 available genomic assemblies of some P. syringae pathovars, as well as 43 genomic assemblies of closely related species P. cannabina and P. savastanoi. The analysis showed a high similarity of most of the analyzed sequences with the sequences of P. c. pv. alisalensis pathovar.
About the Authors
S. I. Prikhodko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Svetlana Prikhodko, Researcher, Head of Bacteriology and GMO Analysis Laboratory, Laboratory Testing Center
Bykovo, Ramenskoye, Moscow Oblast
A. B. Yaremko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Anastasia Yaremko, Junior Researcher, Molecular and Genetic Diagnostic Methods Department
Bykovo, Ramenskoye, Moscow Oblast
E. V. Starikova
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Elizaveta Starikova, Agronomist, Virology Laboratory, Laboratory Testing Center
Bykovo, Ramenskoye, Moscow Oblast
K. P. Kornev
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Konstantin Kornev, PhD in Biology, Deputy Director
Bykovo, Ramenskoye, Moscow Oblast
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Review
For citations:
Prikhodko S.I., Yaremko A.B., Starikova E.V., Kornev K.P. Analysis of genetic differences of some Pseudomonas syringae pathovars. Plant Health and Quarantine. 2022;(2):50-59. https://doi.org/10.69536/s9564-4378-7598-q
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