Diagnosis of Tobamovirus genus viruses infecting Solanaceae
https://doi.org/10.69536/p4517-2518-6750-w
Abstract
Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), Tomato brown rugose fruit virus (ToBRFV), Pepper mild mottle virus (PMMoV), Tobacco mild green mosaic virus (TMGMV) are serious pests of Solanaceae in open and protected ground. Yield losses of tomato and pepper as a result of infection with these pathogens can reach 100%. Early detection of infected seed lots and outbreaks of these viruses is essential to prevent their rapid spread. In the course of the research, the specificity of seven test systems for enzyme immunoassay (ELISA) to TMV, ToMV, TMGMV and PMMoV of leading manufacturers was evaluated. The polymerase chain reaction (PCR) was tested with 21 pairs of universal and species-specific primers. Primers have been identified that allow universal detection of the tobamovirus complex on Solanaceae crops. Two methods of multiplex PCR were tested to detect and identify several viruses simultaneously. The primers were screened for their specificity for TMV, ToMV, TMGMV and PMMoV. Proprietary primers with high specificity for TMV and ToMV were developed.
About the Authors
E. N. Lozovaya
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Evgenia Lozovaya, Researcher of Post-Graduate Studies Department
Bykovo, Ramenskoye, Moscow Oblast
Yu. N. Prikhodko
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuri Prikhodko, PhD in Agriculture, Leading Researcher, Research and Methodology Department of Virology and Bacteriology
Bykovo, Ramenskoye, Moscow Oblast
T. S. Zhivaeva
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Tatiana Zhivaeva, Researcher, Research and Methodology Department of Virology and Bacteriology
Bykovo, Ramenskoye, Moscow Oblast
Yu. A. Shneyder
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Yuri Shneyder, PhD in Biology, Head of Research and Methodology and Experimental Center, Leading Researcher
Bykovo, Ramenskoye, Moscow Oblast
E. V. Karimova
FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)
Russian Federation
Russian Federation
Elena Karimova, PhD in Biology, Senior Researcher, Head of Research and Methodology Department of Virology and Bacteriology
Bykovo, Ramenskoye, Moscow Oblast
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Review
For citations:
Lozovaya E.N., Prikhodko Yu.N., Zhivaeva T.S., Shneyder Yu.A., Karimova E.V. Diagnosis of Tobamovirus genus viruses infecting Solanaceae. Plant Health and Quarantine. 2022;(2):39-49. https://doi.org/10.69536/p4517-2518-6750-w
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