phytosanitaryФитосанитария. Карантин растенийPlant Health and Quarantine2782-327X10.69536/FKR.2021.34.41.001phytosanitary-89Research ArticleНАУЧНЫЕ ИССЛЕДОВАНИЯSCIENTIFIC RESEARCHВирус некроза побегов хризантемы (Chrysanthemum stem necrosis virus): распространенность, биология, методы диагностикиChrysanthemum stem necrosis virus: area, biology, diagnosis methodshttps://orcid.org/0000-0003-1276-6997КорнилаеваО. Н.KornilaevaO. N.

Корнилаева Ольга Николаевна – научный сотрудник – заведующая лабораторией вирусологии.

р. п. Быково, г. Раменское, Московская обл.

Olga Kornilaeva – researcher, head of Virology Laboratory, Testing Laboratory Center.

Bykovo, Ramenskoye, Moscow Oblast

morozova-510@yandex.ruhttps://orcid.org/0000-0002-8564-8142БелошапкинаО. О.BeloshapkinaO. O.

Белошапкина Ольга Олеговна – доктор сельскохозяйственных наук, профессор кафедры защиты растений.

г. Москва

Olga Beloshapkina – Doctor of Agriculture, professor of the Plant Protection Department.

Moscow

beloshapkina58@mail.ruПриходькоЮ. Н.PrikhodkoYu. N.

Приходько Юрий Николаевич – кандидат сельскохозяйственных наук, ведущий научный сотрудник научно-методического отдела вирусологии и бактериологии

р. п. Быково, г. Раменское, Московская обл.

Yuriy Prikhodko – PhD in Agriculture, leading researcher, Research and Methodology Departmet for Virology and Bacteriology

Bykovo, Ramenskoye, Moscow Oblast

prihodko_yuri59@mail.ruФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»РоссияFGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)Russian FederationФГБОУ ВО «Российский государственный аграрный университет – МСXА имени К.А. Тимирязева»РоссияRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian FederationФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»)РоссияFGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)Russian Federation202106012022041623Copyright © Корнилаева О.Н., Белошапкина О.О., Приходько Ю.Н., 20222022Корнилаева О.Н., Белошапкина О.О., Приходько Ю.Н.Kornilaeva O.N., Beloshapkina O.O., Prikhodko Y.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://phytosanitary.vniikr.ru/jour/article/view/89

Вирус некроза побегов хризантемы Chrysanthemum stem necrosis virus (CSNV), распространенный в Южной Корее, Бразилии, Иране и Японии, при сильном заражении вызывает отмирание растений-хозяев, основными из которых являются хризантема крупноцветковая и томат. К эффективным переносчикам вируса относятся калифорнийский и томатный трипсы. Существует реальная опасность интродукции и широкой акклиматизации данного вируса как в теплицах, так и в открытом грунте в южных регионах Российской Федерации, что вызывает необходимость его достоверной диагностики лабораторными методами. Экспериментально на базе ВНИИКР доказано, что метод полимеразной цепной реакции  (ПЦР) в  режиме реального времени оказался более чувствительным методом по сравнению с классической ПЦР. В проанализированных методом ПЦР образцах растений хризантемы из нескольких оранжерейных хозяйств вирус некроза побегов хризантемы не был обнаружен.

Chrysanthemum stem necrosis virus (CSNV), common in South Korea, Brazil, Iran and Japan, with a strong infection causes the death of host plants, the main of which are Dendranthema x grandiflorum and tomato. Effective vectors of the virus are western flower thrips and tomato thrips. There is a real risk of introduction and massive adaptation of this virus both in green – houses and open ground in the southern regions of the Russian Federation, which accounts for the necessity of its reliable diagnosis by laboratory methods. FGBU “VNIIKR” has experimentally proved that the method of polymerase chain reaction in real time (RT-PCR) turned out to be a more sensitive method compared to classical PCR. Chrysanthemum stem necrosis virus was not detected in chrysanthemum plant samples from several greenhouse farms analyzed by PCR.

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The authors declare that there are no conflicts of interest present.