phytosanitaryФитосанитария. Карантин растенийPlant Health and Quarantine2782-327X10.69536/FKR.2020.91.31.001phytosanitary-27Research ArticleНАУЧНЫЕ ИССЛЕДОВАНИЯSCIENTIFIC RESEARCHДиагностика бегомовирусов методом ПЦРDiagnosis of begomoviruses by PCRЛозоваяЕ. Н.LozovaiaE. N.

научный сотрудник отдела аспирантуры

Researcher of the Postgraduate Study Department

evgeniyaf@mail.ruПриходькоЮ. Н.PrikhodkoY. N.

к. с.-х. н., ведущий научный сотрудник НМОВБ

PhD in Agriculture, Leading Researcher of the Research and Methodology Department for Virology and Bacteriology

prihodko_yuri59@mail.ruЖиваеваТ. С.ZhivaevaT. S.

научный сотрудник НМОВБ

Researcher of the Research and Methodology Department for Virology and Bacteriology

zhivaeva.vniikr@mail.ruШнейдерЮ. А.ShneyderY. A.

к. б. н., и. о. заместителя директора

PhD in Biology, Acting Deputy Director

yury.shneyder@mail.ruФГБУ «ВНИИКР»РоссияFGBU “VNIIKR”Russian Federation202002062021124154Copyright © Лозовая Е.Н., Приходько Ю.Н., Живаева Т.С., Шнейдер Ю.А., 20212021Лозовая Е.Н., Приходько Ю.Н., Живаева Т.С., Шнейдер Ю.А.Lozovaia E.N., Prikhodko Y.N., Zhivaeva T.S., Shneyder Y.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://phytosanitary.vniikr.ru/jour/article/view/27

Род Begomovirus включает более 190 видов вирусов, являющихся вредоносными патогенами овощных и полевых культур. Широко распространенный в Европе бегомовирус желтой курчавости листьев томата (Tomato yellow leaf curl virus, TYLCV) является карантинным объектом для Российской Федерации, ЕАЭС и ЕОКЗР. Идентификация бегомовирусов до видового уровня сопряжена с определенными трудностями, обусловленными высокой идентичностью их генома. В статье приводятся результаты испытания 19 пар универсальных и специфических праймеров для диагностики бегомовирусов: вируса желтой курчавости листьев томата TYLCV, вируса желтой курчавости листьев томата Сардиния (Tomato yellow leaf curl Sardinia virus, TYLCSV) и Нью-Дели вируса курчавости листьев томата (Tomato leaf curl New Delhi virus, ToLCNDV) методами классической ПЦР и ПЦР в реальном времени.

The genus Begomovirus includes more than 190 species of viruses, which are harmful pathogens of vegetable and field crops. Tomato yellow leaf curl virus (TYLCV), which is widely spread in Europe, is a quarantine object for the Russian Federation, EAEU and EPPO. Identifying begomoviruses to the species level involves certain difficulties due to the high identity of their genome. The article presents the results of testing 19 pairs of universal and specific primers for the diagnosis of begomoviruses: tomato leaf curl virus (TYLCV), tomato yellow leaf curl Sardinia virus (TYLCSV), and tomato leaf curl New Delhi virus (ToLCNDV) by conventional and real-time PCR methods.

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The authors declare that there are no conflicts of interest present.