phytosanitaryФитосанитария. Карантин растенийPlant Health and Quarantine2782-327X10.69536/FKR.2020.86.74.001phytosanitary-26Research ArticleНАУЧНЫЕ ИССЛЕДОВАНИЯSCIENTIFIC RESEARCHИдентификация грибов рода Monilinia при фитосанитарной диагностикеIdentification of fungi of genus Monilinia during phytosanitary diagnosisШухинД. И.ShukhinD. I.

младший научный сотрудник лаборатории микологии Испытательного лабораторного центра

Junior Researcher of the Mycology Laboratory of Laboratory Testing Center

dmitriq.shukhin@gmail.comФГБУ «ВНИИКР»РоссияFGBU “VNIIKR”Russian Federation202002062021123340Copyright © Шухин Д.И., 20212021Шухин Д.И.Shukhin D.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://phytosanitary.vniikr.ru/jour/article/view/26

В статье приведены традиционные, а также современные методы идентификации возбудителей монилиозов плодовых культур – грибов рода Monilinia, используемые при фитосанитарной диагностике, такие как классическая ПЦР и ПЦР в реальном времени, а также мультиплексная ПЦР. Описан новый метод масс-спектрометрии MALDI-ToF MS, а также методы выявления патогенов при латентной инфекции.

The article presents traditional, as well as modern techniques of identification of fruit crop moniliosis – fungi of the genus Monilinia – used in phytosanitary diagnosis, such as conventional PCR, qPCR, and multiplex PCR. The new MALDI-ToF MS mass spectrometry technique is described, as well as techniques of latent pathogen infection detection.

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The authors declare that there are no conflicts of interest present.