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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">phytosanitary</journal-id><journal-title-group><journal-title xml:lang="ru">Фитосанитария. Карантин растений</journal-title><trans-title-group xml:lang="en"><trans-title>Plant Health and Quarantine</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2782-327X</issn><publisher><publisher-name></publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.69536/8237.2024.86.86.002</article-id><article-id custom-type="elpub" pub-id-type="custom">phytosanitary-159</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МОРФОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MORPHOLOGY</subject></subj-group></article-categories><title-group><article-title>Культурально-морфологические особенности видов рода Diaporthe при фитосанитарных исследованиях растений рода Vaccinium</article-title><trans-title-group xml:lang="en"><trans-title>Cultural and morphological features of Diaporthe spp. in phytosanitary diagnostics of Vaccinium plants</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8443-2641</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кузнецова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kuznetsova</surname><given-names>Anna A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кузнецова Анна Александровна, старший научный сотрудник научно-методического отдела микологии,</p><p>р. п. Быково, г. о. Раменский, Московская область.</p></bio><bio xml:lang="en"><p>Anna Kuznetsova, Senior Researcher, Research and Methodology Mycology and Helminthology Department,</p><p>Bykovo, Ramenskoye, Moscow Oblast.</p></bio><email xlink:type="simple">kyyznec@bk.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4334-9224</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Цветкова</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Tsvetkova</surname><given-names>Yulia V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Цветкова Юлия Владиславовна, младший научный сотрудник лаборатории микологии ИЛЦ; аспирант,</p><p>р. п. Быково, г. о. Раменский, Московская область;   </p><p>г. Москва.</p></bio><bio xml:lang="en"><p>Yulia Tsvetkova, Junior Researcher, Mycology Laboratory, Testing Laboratory Center; PhD student,</p><p>Bykovo, Ramenskoye, Moscow Oblast;</p><p>Moscow</p></bio><email xlink:type="simple">yutska@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0005-1164-7373</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Синкевич</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Sinkevich</surname><given-names>Olga V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Синкевич Ольга Владимировна, кандидат сельскохозяйственных наук, научный сотрудник,</p><p>г. Петрозаводск, Республика Карелия, 185030 .</p></bio><bio xml:lang="en"><p>Olga Sinkevich, PhD in Agriculture, Researcher,</p><p>Petrozavodsk, Republic of Karelia.</p></bio><email xlink:type="simple">ovbio@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0003-8066-0753</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Костин</surname><given-names>Н. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Kostin</surname><given-names>Nikita K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Костин Никита Константинович, младший научный сотрудник научно-методического отдела микологии; студент,</p><p>р. п. Быково, г.о. Раменский, Московская область; </p><p>г. Москва.</p></bio><bio xml:lang="en"><p>Nikita Kostin, Junior Researcher, Research and Methodology Mycology and Helminthology Department; Student</p><p>Bykovo, Ramenskoye, Moscow Oblast;</p><p>Moscow.</p></bio><email xlink:type="simple">kostinwork1@gmail.com</email><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal State Budgetary Institution “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»); Московский государственный университет им. М. В. Ломоносова, Биологический факультет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal State Budgetary Institution “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”); Lomonosov Moscow State University, Biology Faculty</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Североморский филиал ФГБУ «Всероссийский центр карантина растений»(ФГБУ «ВНИИКР»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FGBU “VNIIKR”, Severomorsk branch</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>ФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»); Российский государственный агарный университет – МСХА им. К.А. Тимирязева («РГАУ-МСХА»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal State Budgetary Institution “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”); Moscow Timiryazev Agricultural Academy</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>29</day><month>05</month><year>2024</year></pub-date><volume>0</volume><issue>2</issue><fpage>29</fpage><lpage>40</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кузнецова А.А., Цветкова Ю.В., Синкевич О.В., Костин Н.К., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Кузнецова А.А., Цветкова Ю.В., Синкевич О.В., Костин Н.К.</copyright-holder><copyright-holder xml:lang="en">Kuznetsova A.A., Tsvetkova Y.V., Sinkevich O.V., Kostin N.K.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://phytosanitary.vniikr.ru/jour/article/view/159">https://phytosanitary.vniikr.ru/jour/article/view/159</self-uri><abstract><p>Большинство видов рода Diaporthe относятся к фитопатогенным грибам, поражающим широкий круг растений-хозяев, в том числе представителей рода Vaccinium. Данные грибы зачастую являются причиной апикального некроза стеблей, вызывающего усыхание и гибель растений, а также существенно влияющего на лежкость и товарный вид ягод. Возбудители имеют сходные симптомы проявления болезни на растениях, а при выделении в чистую культуру - похожие культурально-морфологические признаки, что затрудняет проведение достоверной фитосанитарной диагностики. Следует отметить, что один из видов рода Diaporthe vaccinii Shear имеет карантинный статус для Российской Федерации. В данной работе проведено исследование таких видов рода Diaporthe как Diaporthe eres Nitschke, Diaporthe rudis Nitschke и Diaporthe bohemiae Guarnaccia, Eichmeier &amp; Crous, полученных при обследовании насаждений голубики высокорослой (V. corymbosum L.) из Калининградской области, и карантинного вида D. vaccinii, выделенного при фитосанитарном исследовании посадочного материала клюквы крупноплодной (V. macrocarpon L.) импортного происхождения (Беларусь). Вид D. bohemiae впервые был обнаружен в России в образцах голубики высокорослой из Калининградской области. В результате исследований получен метод, ускоряющий образование и развитие пикнид и пикноспор, представленных в виде 2-х типов спор: альфа (α) и бета (β) конидий, тестируемых видов рода Diaporthe. При помощи культивирования изолятов на 2% солодовом агаре с дополнительным досвечиванием эритемной лампой в течение 7 дней были получены диагностически значимые морфологические структуры D. vaccinii. Метод применим при лабораторном исследовании подкарантинного материала растений рода Vaccinium для выявления и идентификации возбудителя вязкой гнили черники D. vaccinii. В ходе работы были описаны культурально-морфологические особенности и приведена сравнительная характеристика исследуемых видов D. vaccinii, D. eres, D. rudis и D. bohemiae.</p></abstract><trans-abstract xml:lang="en"><p>Most Diaporthe spp. belong to phytopathogenic fungi affecting a wide range of host plants, including the genus Vaccinium. These fungi often cause apical necrosis of stems, leading to plant drying out and death, and also significantly affecting the shelf life and presentation of berries. The pathogens have similar symptoms of disease manifestation on plants, and when isolated into a pure culture, they have similar cultural and morphological features, which makes it difficult to carry out reliable phytosanitary diagnostics. It should be noted that one of the species of the genus Diaporthe vaccinii Shear has a quarantine status for the Russian Federation. In this work, we conducted a study of species of the genus Diaporthe such as Diaporthe eres Nitschke, Diaporthe rudis Nitschke and Diaporthe bohemiae Guarnaccia, Eichmeier &amp; Crous, obtained during the examination of the northern highbush blueberry plantings (V. corymbosum L.) from Kaliningrad Oblast, and the quarantine species D. vaccinii, isolated during phytosanitary examination of large cranberry planting material (V. macrocarpon L.) of imported origin (Belarus). D. bohemiae was first detected in Russia in samples of highbush blueberry from Kaliningrad Oblast. As a result of the research, a method was obtained that accelerates the formation and development of pycnidia and pycnospores, presented in the form of 2 spore types: alpha (α) and beta (β) conidia, tested species of the genus Diaporthe. By cultivating isolates on 2% malt agar with additional illumination with an erythema lamp for 7 days, diagnostically significant morphological structures of D. vaccinii were obtained. The method is applicable in laboratory research of regulated material of plants of the genus Vaccinium for the detection and identification of the causative agent of viscid rot of blueberries D. vaccinii. During the work, cultural and morphological features were described and comparative characteristics of the studied species D. vaccinii, D. eres, D. rudis and D. bohemiae were given.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>вязкая гниль черники</kwd><kwd>фомопсисы</kwd><kwd>диагностика</kwd><kwd>выявление и идентификация</kwd><kwd>досвечивание</kwd></kwd-group><kwd-group xml:lang="en"><kwd>viscid rot of blueberries</kwd><kwd>phomopsis</kwd><kwd>diagnostics</kwd><kwd>detection and identification</kwd><kwd>supplementary illumination</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Ali S. et al. 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