phytosanitaryФитосанитария. Карантин растенийPlant Health and Quarantine2782-327X10.69536/p4517-2518-6750-wphytosanitary-104Research ArticleНАУЧНЫЕ ИССЛЕДОВАНИЯSCIENTIFIC RESEARCHДиагностика вирусов рода Tobamovirus, заражающих пасленовые овощные культурыDiagnosis of Tobamovirus genus viruses infecting SolanaceaeЛозоваяЕ. Н.LozovayaE. N.

Лозовая Евгения Николаевна, научный сотрудник отдела аспирантуры

р. п. Быково, г. Раменское, Московская обл.

Evgenia Lozovaya, Researcher of Post-Graduate Studies Department

Bykovo, Ramenskoye, Moscow Oblast

evgeniyaf@mail.ruПриходькоЮ. Н.PrikhodkoYu. N.

Приходько Юрий Николаевич, кандидат сельскохозяйственных наук, ведущий научный сотрудник научно-методического отдела вирусологии и бактериологии

р. п. Быково, г. Раменское, Московская обл.

Yuri Prikhodko, PhD in Agriculture, Leading Researcher, Research and Methodology Department of Virology and Bacteriology

Bykovo, Ramenskoye, Moscow Oblast

prihodko_yuri59@mail.ruЖиваеваТ. С.ZhivaevaT. S.

Живаева Татьяна Степановна, научный сотрудник научно-методического отдела вирусологии и бактериологии

р. п. Быково, г. Раменское, Московская обл.

 

Tatiana Zhivaeva, Researcher, Research and Methodology Department of Virology and Bacteriology

Bykovo, Ramenskoye, Moscow Oblast

zhivaeva.vniikr@mail.ruhttps://orcid.org/0000-0002-7565-1241ШнейдерЮ, А.ShneyderYu. A.

Шнейдер Юрий Андреевич, кандидат биологических наук, начальник научно-методического и экспериментального центра, ведущий научный сотрудник

р. п. Быково, г. Раменское, Московская обл.

Yuri Shneyder, PhD in Biology, Head of Research and Methodology and Experimental Center, Leading Researcher

Bykovo, Ramenskoye, Moscow Oblast

yury.shneyder@mail.ruhttps://orcid.org/0000-0001-6474-8913КаримоваЕ. В.KarimovaE. V.

Каримова Елена Владимировна, кандидат биологических наук, старший научный сотрудник – начальник научно-методического отдела вирусологии и бактериологии

р. п. Быково, г. Раменское, Московская обл.

Elena Karimova, PhD in Biology, Senior Researcher, Head of Research and Methodology Department of Virology and Bacteriology

Bykovo, Ramenskoye, Moscow Oblast

elenavkar@mail.ruФГБУ «Всероссийский центр карантина растений (ФГБУ «ВНИИКР»)РоссияFGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)Russian Federation202209062022023949Copyright © Лозовая Е.Н., Приходько Ю.Н., Живаева Т.С., Шнейдер Ю.А., Каримова Е.В., 20222022Лозовая Е.Н., Приходько Ю.Н., Живаева Т.С., Шнейдер Ю.А., Каримова Е.В.Lozovaya E.N., Prikhodko Y.N., Zhivaeva T.S., Shneyder Y.A., Karimova E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://phytosanitary.vniikr.ru/jour/article/view/104

Тобамовирусы мозаики томата (ToMV), табачной мозаики (TMV), коричневой морщинистости плодов томата (ToBRFV), слабой крапчатости перца (PMMoV), слабой зеленой мозаики табака (TMGMV) являются опасными патогенами пасленовых овощных культур в открытом и защищенном грунте. Потери урожая томата и перца в результате заражения этими патогенами могут достигать 100%. Раннее выявление зараженных партий семян и очагов этих вирусов имеет важнейшее значение для предотвращения их быстрого распространения. В ходе исследований выполнена оценка специфичности семи тест-систем для иммуноферментного анализа (ИФА) к TMV, ToMV, TMGMV и PMMoV ведущих фирм-производителей. Проведена отработка полимеразной цепной реакции (ПЦР) с 21 парой универсальных и видоспецифичных праймеров. Определены праймеры, позволяющие проводить универсальное выявление комплекса тобамовирусов на пасленовых овощных культурах. Проведена отработка двух методик мультиплексной ПЦР для выявления и идентификации нескольких вирусов одновременно. Осуществлен скрининг праймеров в отношении их специфичности к TMV, ToMV, TMGMV и PMMoV. Разработаны собственные праймеры, характеризующиеся высокой специфичностью к TMV и ToMV.

Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), Tomato brown rugose fruit virus (ToBRFV), Pepper mild mottle virus (PMMoV), Tobacco mild green mosaic virus (TMGMV) are serious pests of Solanaceae in open and protected ground. Yield losses of tomato and pepper as a result of infection with these pathogens can reach 100%. Early detection of infected seed lots and outbreaks of these viruses is essential to prevent their rapid spread. In the course of the research, the specificity of seven test systems for enzyme immunoassay (ELISA) to TMV, ToMV, TMGMV and PMMoV of  leading manufacturers was evaluated. The polymerase chain reaction (PCR) was tested with 21 pairs of universal and species-specific primers. Primers have been identified that allow universal detection of the tobamovirus complex on Solanaceae crops. Two methods of multiplex PCR were tested to detect and identify several viruses simultaneously. The primers were screened for their specificity for TMV, ToMV, TMGMV and PMMoV. Proprietary primers with high specificity for TMV and ToMV were developed.

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The authors declare that there are no conflicts of interest present.