<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">phytosanitary</journal-id><journal-title-group><journal-title xml:lang="ru">Фитосанитария. Карантин растений</journal-title><trans-title-group xml:lang="en"><trans-title>Plant Health and Quarantine</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2782-327X</issn><publisher><publisher-name></publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.69536/k2355-2320-4825-k</article-id><article-id custom-type="elpub" pub-id-type="custom">phytosanitary-103</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>НАУЧНЫЕ ИССЛЕДОВАНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>SCIENTIFIC RESEARCH</subject></subj-group></article-categories><title-group><article-title>Заболевание пасмо льна. Биологический метод идентификации Septoria linicola</article-title><trans-title-group xml:lang="en"><trans-title>Pasmo disease of flax. Biological method of identification of Septoria linicola</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0169-414X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дудченко</surname><given-names>И. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Dudchenko</surname><given-names>I. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Дудченко Ирина Петровна, старший научный сотрудник научно-методического отдела микологии и гельминтологии</p><p>р. п. Быково, г. Раменское, Московская обл.</p></bio><bio xml:lang="en"><p>Irina Dudchenko, Senior Researcher, Mycology and Helminthology Research and Methodology Department</p><p>Bykovo, Ramenskoye, Moscow Oblast</p></bio><email xlink:type="simple">dudchenko_irina@vniikr.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9787-8351</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Щуковская</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Schukovskaya</surname><given-names>A. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Щуковская Анастасия Геннадиевна, кандидат биологических наук, старший научный сотрудник отдела лесного карантина</p><p>р. п. Быково, г. Раменское, Московская обл.</p></bio><bio xml:lang="en"><p>Anastasia Schukovskaya, PhD in Biology, Senior Researcher, Forest Quarantine Department</p><p>Bykovo, Ramenskoye, Moscow Oblast</p></bio><email xlink:type="simple">schukovskaya.a@vniikr.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дудченко</surname><given-names>Г. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Dudchenko</surname><given-names>G. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Дудченко Геннадий Николаевич, агроном редакционно-издательского отдела</p><p>р. п. Быково, г. Раменское, Московская обл.</p></bio><bio xml:lang="en"><p>Gennady Dudchenko, Agronomist, Editorial and Publishing Department</p><p>Bykovo, Ramenskoye, Moscow Oblast</p></bio><email xlink:type="simple">dudchenko_gennadiy@vniikr.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБУ «Всероссийский центр карантина растений» (ФГБУ «ВНИИКР»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FGBU “All-Russian Plant Quarantine Center” (FGBU “VNIIKR”)</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>09</day><month>06</month><year>2022</year></pub-date><volume>0</volume><issue>2</issue><fpage>25</fpage><lpage>38</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Дудченко И.П., Щуковская А.Г., Дудченко Г.Н., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Дудченко И.П., Щуковская А.Г., Дудченко Г.Н.</copyright-holder><copyright-holder xml:lang="en">Dudchenko I.P., Schukovskaya A.G., Dudchenko G.N.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://phytosanitary.vniikr.ru/jour/article/view/103">https://phytosanitary.vniikr.ru/jour/article/view/103</self-uri><abstract><p>Пасмо является опасным заболеванием для культурных и дикорастущих видов льна, и его возбудитель, Mycosphaerella linicola Naumov (Septoria linicola (Speg.) Garass.), имеет карантинный статус для ряда стран, закупающих льнопродукцию в России. Для того чтобы экспортируемая из России семенная продукция льна соответствовала современным фитосанитарным требованиям, она должна быть свободна от опасных растительных патогенов, в том числе и от Mycosphaerella linicola, который, несмотря на широкое распространение, входит в карантинные списки таких стран, как Египет, Узбекистан, Республика Корея, Китай и Беларусь. Поэтому понимание биологических процессов жизнедеятельности возбудителя пасмо льна и связанные с этим вопросы обнаружения и идентификации приобретают особое значение. Современная диагностика фитопатогенов включает в себя комплекс мер, основанных на сочетании молекулярно-генетических и классических методов. В статье рассматриваются вопросы, относящиеся к биологическому методу идентификации возбудителя, основанному на знаниях биологических особенностей развития заболевания и  культурально-морфологического анализа. В исследованиях, проведенных авторами в  2021 г. на базе ФГБУ «ВНИИКР», выявлены и наглядно проиллюстрированы важные аспекты прохождения жизненного цикла Septoria linicola на агаризованных питательных средах, позволяющие идентифицировать возбудителя пасмо льна в лабораторных условиях в короткие сроки. Также в статье рассматриваются вопросы истории распространения заболевания и изменений таксономического статуса возбудителя.</p></abstract><trans-abstract xml:lang="en"><p>Pasmo is a dangerous disease for cultivated and wild flax species, its pathogen, Mycosphaerella linicola Naumov (Septoria linicola (Speg.) Garass.), has a quarantine status for some countries importing flax products from Russia. For flax seed products exported from Russia to meet modern phytosanitary requirements, they must be free from dangerous plant pathogens, including Mycosphaerella linicola, which, despite its wide distribution, is included in the quarantine lists of countries such as Egypt, Uzbekistan, the Republic of Korea, China and Belarus. Therefore, understanding the biological processes of the vital activity of the pasmo flax disease pathogen and the related issues of detection and identification are of particular importance. Modern diagnostics of phytopathogens includes a set of measures based on a combination of molecular genetic and classical methods. The article considers issues related to the biological method of pathogen identification, based on knowledge of the biological characteristics of the disease development and cultural and morphological analysis. In the studies conducted by the authors in 2021 on the basis of FGBU “VNIIKR”, important aspects of the life cycle of Septoria linicola are identified and clearly illustrated on agar nutrient media, allowing identification of the causative agent of flax pasmo disease in the laboratory in a short time. The article also discusses the history of the spread of the disease and changes in the taxonomic status of the pathogen.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>мycosphaerella linicola</kwd><kwd>стадия Septogloeum</kwd><kwd>спороношение типа Melanconiales</kwd><kwd>пикнидиальное спороношение</kwd><kwd>культурально-морфологический метод</kwd><kwd>чистая культура</kwd><kwd>псевдотеции</kwd><kwd>аскоспоры</kwd><kwd>физиологическая форма</kwd><kwd>инокуляция</kwd><kwd>диагностика</kwd></kwd-group><kwd-group xml:lang="en"><kwd>мycosphaerella linicola</kwd><kwd>Septogloeum stage</kwd><kwd>Melanconiales sporulation</kwd><kwd>pycnidial sporulation</kwd><kwd>cultural morphological method</kwd><kwd>pure culture</kwd><kwd>pseudothecia</kwd><kwd>ascospores</kwd><kwd>physiological form</kwd><kwd>inoculation</kwd><kwd>diagnostics</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Васильевский Н., 1924. К биологии Septoria ribis Desm. на черной смородине. – Журнал «Болезни растений», № 1: 10–14.</mixed-citation><mixed-citation xml:lang="en">Vasilievsky N. On the biology of Septoria ribis Desm. on black currant [K biologii Septoria ribis Desm. na chernoy smorodine]. Plant diseases, 1924; 1: 10–14 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Вахрушева Т., 1985. Изучение сортовой однородности льна к пасмо. – Бюллетень ВИР, № 154: 63–65.</mixed-citation><mixed-citation xml:lang="en">Vakhrusheva T. The study of varietal uniformity of flax to pasmo disease [Izucheniye sortovoy odnorodnosti lna k pasmo]. VIR Bulletin, 1985; 154: 63–65 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Курчакова Л. Эколого-генетические аспекты устойчивости льна к септориозу (пасмо) в селекции льна-долгунца: дисс. д-ра. с.-х. наук: 06.01.05. – М., 2009. 315 с.</mixed-citation><mixed-citation xml:lang="en">Kurchakova L. Ecological and genetic aspects of flax resistance to Septoria (pasmo) in fiber flax breeding [Ekologo-geneticheskiye aspekty ustoychivosti lna k septoriozu (pasmo) v selektsii lna-dolguntsa]: diss. PhD in Agriculture: 06.01.05. M. 2009: 315 p. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Натальина О., 1931. Предварительное сообщение о болезни льна, вызываемой грибком Phlyctaena linicola S. и обнаруженной на Дальнем Востоке летом 1930 г. – Защита растений, VIII, 2: 177–182.</mixed-citation><mixed-citation xml:lang="en">Natalina O. Preliminary report on flax disease caused by the fungus Phlyctaena linicola S., and discovered in the Far East in the summer of 1930 [Predvaritelnoye soobshcheniye o bolezni lna, vyzyvayemoy gribkom Phlyctaena linicola S. i obnaruzhennoy na Dalnem Vostoke letom 1930]. Plant protection, 1931; VIII, 2: 177–182 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Примаковская М., 1971. Пасмо льна. – Защита растений, № 2: 49–50.</mixed-citation><mixed-citation xml:lang="en">Primakovskaya M. Pasmo disease of flax [Pasmo lna]. Plant protection, 1971; 2: 49–50 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Проценко Е., 1964. Сборник по карантину растений. – М.: Колос, 1964. № 16: 5–63.</mixed-citation><mixed-citation xml:lang="en">Protsenko E. Collection of plant quarantine [Sbornik po karantinu rasteniy]. M. Kolos, 1964; 16: 5–63 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Anselme C., 1960. Recherches sur le pasmo des lines a huile. – Ann. Epiphyt., 2 (11): 201–215.</mixed-citation><mixed-citation xml:lang="en">Anselme C. Recherches sur le pasmo des lines a huile. Ann. Epiphyt., 1960; 2 (11): 201–215.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Garassini L., 1938. Ubicacion generica del micromicete que produce el “pasmo” del lino. – Revista de la Fac. de Agronomia de La-Plata, Vol. 23: 45.</mixed-citation><mixed-citation xml:lang="en">Garassini L. Ubicacion generica del micromicete que produce el “pasmo” del lino. Revista de la Fac. de Agronomia de La-Plata, 1938; 23: 45.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Harris H., 1935. Morphologiс studies of Septoria lycopersici. – Phytopathology, 25: 790–799.</mixed-citation><mixed-citation xml:lang="en">Harris H. Morphologiс studies of Septoria lycopersici. Phytopathology, 1935; 25: 790–799.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Paumier D. et al., 2020. First report ofthe sexual stage oftheflax pathogen Mycosphaerella linicola inFrance andits impact onpasmo epidemiology. – Plantpathology, 70 (2). URL: https://doi.org/10.1101/2020.06.17.156984.</mixed-citation><mixed-citation xml:lang="en">Paumier D. et al. First report of the sexual stage of the flax pathogen Mycosphaerella linicola in France and its impact on pasmo epidemiology. Plant pathology, 2020; 70 (2). URL: https://doi.org/10.1101/2020.06.17.156984.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Rataj K., 1953. Septoria linicola (Speg.) Gar. v Ceskoslovensku. – Sbornik Ceskoclovenske Akademic. Zemedelskyck. Ved, № 3: 229–243.</mixed-citation><mixed-citation xml:lang="en">Rataj K. Septoria linicola (Speg.) Gar. v Ceskoslovensku. Sbornik Ceskoclovenske Akademic. Zemedelskyck. Ved, 1953; 3: 229–243.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Spegazzini C., 1911. Phtyctena linicola Speg. (n. f.). Mycetes Argentinenses. – Annales del Museo Nacional de Buenos Aires. Ser. III, 13: 389–390.</mixed-citation><mixed-citation xml:lang="en">Spegazzini C. Phtyctena linicola Speg. (n. f.). Mycetes Argentinenses. Annales del Museo Nacional de Buenos Aires. 1911; III, 13: 389–390.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Spegazzini C., 1911. Mycetes Argentinenses (Series V). – Anales del Museo Nacional de Historia Natural Buenos Aires. Series III, 13: 329–467.</mixed-citation><mixed-citation xml:lang="en">Spegazzini C. Mycetes Argentinenses (Series V). Anales del Museo Nacional de Historia Natural Buenos Aires. 1911; III, 13: 329–467.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">WollenweberH., 1938. “Sphaerelle linicola” n. sp. Die UrsachederAmerikanischer Leinpest. (Pasmo – oder “Septoria” – Krankheit). – Revista deBotanica delInstituto Miguel Lillo, Lilloa. Tucuman Argentina, 2: 483–495.</mixed-citation><mixed-citation xml:lang="en">Wollenweber H. “Sphaerelle linicola” n. sp. Die Ursache der Amerikanischer Leinpest (Pasmo – oder “Septoria” – Krankheit). Revista deBotanica delInstituto Miguel Lillo, Lilloa. Tucuman Argentina, 1938; 2: 483–495.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Пасмо льна [Электронный ресурс]. – URL: https://betaren.ru/harmful/bolezni/bolezni-lna/pasmo/ (дата обращения: 04.03.2022).</mixed-citation><mixed-citation xml:lang="en">Pasmo disease of flax [Electronic resource]. URL: https://betaren.ru/harmful/bolezni/bolezni</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
